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nicking reaction catalysed from the MobA89K and MobC89K proteins. The cleaved strand is then unwound through the helicase and sent to the 89K-encoded GI-type T4SS equipment. The VirB1-89K element is assumed to work as a lytic transglycosylase, regionally opening the peptidoglycan with contributions from some other variable(s) throughout the assembl